Skip to main content
. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Chembiochem. 2012 Jul 17;13(12):1818–1825. doi: 10.1002/cbic.201100792

Figure 3. The o-quinone can be intercepted using Besthorn’s reagent.

Figure 3

A) eDHFR-HA (1.5 mg/ml) was treated with m-tyrosinase (0.15 mg/ml) and Besthorn’s reagent (30μM) (left). In the control eDHFR-HA (1.5 mg/ml) was treated with m-tyrosinase (0.15 mg/ml) only (right). Aliquots were analyzed by SDS-PAGE without staining. B) The same gel was stained with Comassie. Apparent gain of mass in lanes 2–3 is probably due to Besthorn’s addition (2 x Besthorn’s ~ 400 Da; 3 x Besthorn’s ~ 600 Da). Note the change in mass is small relative to 0 time point in lane 4. A) is shown in color in Figure S14 G).