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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Chembiochem. 2012 Jul 17;13(12):1818–1825. doi: 10.1002/cbic.201100792

Figure 4. Mutagenesis experiments show that cleavage requires 1st and 3rd tyrosine in HA-tag.

Figure 4

A) eDHFR proteins bearing C-terminal mutant HA-tags used in this study (ND = not determined). B) Representative Coomassie stained SDS-PAGE gel for action of m-tyrosinase (0.15 mg/ml) on mutants (1.5 mg/ml). C) Quantitation of cleavage of all mutants (from Coomassie stained SDS-PAGE gel).

NOTE only YYY and YFY show cleavage: all other mutants lie on X-axis (no cleavage). Conditions as per B). NOTE: In these samples trace BSA (residual from desalting column) was present.