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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Chembiochem. 2012 Jul 17;13(12):1818–1825. doi: 10.1002/cbic.201100792

Figure 5. Fusion proteins in which the HA-tag acts as a linker are substrates for m-tyrosinase and the product is oxidized.

Figure 5

A) GFP-TEV-HA-GST-α1 (1.5 mg/ml) was treated with m-tyrosinase (0.15 mg/ml) and aliquots were extracted at the stated time points. These time points were analyzed using antibodies for HA, GFP and GST-α1 and also stained using Coomassie. B) As in A) but the progress was analyzed by Oxyblot(R), where sample is treated with dinitrophenylhydrazine to produce the hydrazone derivative that is subsesquently recognized by anti-dintrophenyl antibody.