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Functionalization of HA-tag using m-tyrosinase and Besthorn’s reagent is selective in E. coli lysates overexpressing a HA-tagged enzyme.
A) BL21 (DE3) E. coli lysate over expressing eDHFR-HA forms a single pink band when treated with m-tyrosinase (0.15 mg/ml) and Besthorn’s reagent (30 μM) and analyzed by SDS PAGE without staining. This gel is shown in color in Figure S13 A. B) Gel in A) stained with Coomassie.
