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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Chembiochem. 2012 Jul 17;13(12):1818–1825. doi: 10.1002/cbic.201100792

Figure 7. Cross-linking can be favored by using high substrate concentrations or by increasing the number of tyrosines within the tag.

Figure 7

A) eDHFR-HA was added to m-tyrosinase (0.15 mg/ml) and analyzed by SDS-PAGE as a function of time (left); B) eDHFR-(GY)5 (1.5 mg/ml) was added to m-tyrosinase (0.15 mg/ml). C) eDHFR-(GY)5 (1.5 mg/ml) was added to m-tyrosinase (0.15 mg/ml) and after 20 seconds Besthorn’s reagent (30 μM) was added (left) or DMSO was added (right). Time points were extracted and analyzed by SDS-PAGE without staining. D) gel from C) stained using Coomassie.