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Levels of secreted cytokines after five days of recall stimulation with 0.1 cfu ffLVS/PBMC in cell samples from naïve and from LVS vaccinated donors (black bars: donors vaccinated 27-34 years ago; grey bars: donors vaccinated less than three years ago). Cytokines were measured using a 17-plex Bio-Plex kit. Mean values ± SEM of samples from 13-16 individuals per group are presented. P-values were determined using Wilcoxon's test (* P<0.05, ** P<0.01; *** P<0.005).
