Table 2.
Tubulin form | Tub2-BBBBB | Tub2-YYYYY |
---|---|---|
Polymerization Rate (μm/min) | 1.4 ± 0.7 (30) | 1.7 ± 0.7 (33) |
Depolymerization Rate (μm/min) | 1.9 ± 0.7 (38)* | 2.6 ± 1.2 (38) |
Catastrophe Frequency (per min) | 0.59 (28) | 0.64 (29) |
Rescue Frequency (per min) | 1.07 (21) | 1.33 (25) |
Time spent polymerizing, % | 24 | 29 |
Time spent depolymerizing, % | 29 | 29 |
Time spent attenuated, % | 46 | 42 |
polymerization duration, s | 33 ± 13 (30) | 34 ± 16 (33) |
depolymerization duration, s | 31 ± 15 (38) | 30 ± 15 (33) |
attenuation duration, s | 44 ± 28 (44)* | 34 ± 16 (49) |
total microtubules observed | 28 | 28 |
total time observed, s | 4014 | 3858 |
Number of events is in parentheses. Rates/durations are presented as mean ± SD.
p < 0.05 compared to Tub2-YYYYY by unpaired, two-tailed t-test.