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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Sep 20;51(42):10600–10604. doi: 10.1002/anie.201205352

Figure 2.

Figure 2

Selective labeling of the CpK-encoded myoglobin via photoclick chemistry. (a) Coomassie blue stain (bottom panel) and inverted fluorescence image (top panel) of the protein gel after the reaction mixtures were resolved by SDS-PAGE. For photoclick reaction, a solution of Myo-CpK (0.5 mg/mL) and tetrazole 3 (100 μM) in PBS buffer was exposed to 302-nm photoirradiation for a period of 1–12 min. (b) MS2 spectrum of the N-terminal peptide fragment of the pyrazoline-modified myoglobin (Myo-pyr) containing the pyrazoline-linked lysine (K*).