Table 1.
Summary of two-step database searching method for salivary metaproteomics
| Number of RAW files |
Total number of MS/MS spectra |
First stepa | Second stepb | ||
|---|---|---|---|---|---|
| Total sequences in initial database |
Non-human proteins identified |
Total sequences in refined target-decoy database |
Non- human, distinct peptides identified |
||
| 200 | 988,974 | 1,687,426 | 2176 | 152,724 | 1927 |
a
Database used contains human proteins + translated sequences from HOMD. Proteins identified at a low stringency 66% confidence value in ProteinPilot.
b
Database contains human proteins + microbial sequences identified from first step. Peptides were identified using a stringent 5% local FDR value in ProteinPilot.