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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Eur J Immunol. 2009 Sep;39(9):2371–2376. doi: 10.1002/eji.200939536

Figure 3.

Figure 3

The peptide repertoire is synergistically affected by the lack of tapasin membrane association and ERp57 recruitment. A) HLA-B*4402 in cells expressing tapasin lacking membrane and/or ERp57 association has decreased thermostability. After heating the cells at the indicated temperatures, surface HLA-ABC was quantitated using the mAb w6/32 on the live-gated population. The means of four experiments are shown (+/− SEM). B) Antigen presentation is impaired in cells expressing tapasin lacking membrane and/or ERp57 association. 220 cells expressing HLA-B*4402 and WT or mutant tapasins were transfected with constructs encoding MUM-1 or MAGE-A1 antigenic peptides and cultured with the CTL recognizing the corresponding HLA-B*4402/peptide complex. After 20 hrs, IFN-γ was measured in the supernatants. Mean values of four replicates are shown (+/−SEM).