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Spectra acquired in 2.5% MeOH and water and NEt4OH (2:1 NEt4OH:LG). Emission spectra of 5 μM free LG (red) and 5 μM Pb2+ bound LG (black). Excitation for free LG provided at 415 nm. Excitation for Pb2+ bound LG (1:2 Pb2+:LG) provided at 389 nm. Emission maximum observed at 423 nm with a 5-fold increase in emission intensity. Inset is a magnification of free LG (5 μM) emission spectra.
