Fig. 2.

Deleting the individual MHCK genes does not affect the efficiency of chemotaxis in a spatial gradient of cAMP. Chemotaxis was assessed in a gradient chamber designed after that of Zigmond (Zigmond, 1977; Varnum and Soll, 1984). (A–D) Representative perimeter tracks of cells of parental strain JH10 and mutants mhckA⁻, mhckB⁻ and mhckC⁻, respectively. (E) Quantification of motility and chemotaxis parameters. The motility parameters are defined in the legend in Fig. 1. The chemotaxis parameters were chemotactic index (Chem. Index, C.I.) and percentage of cells with a positive chemotactic index (Percent + C.I.). Large arrows in A–D represent direction of cAMP gradient.