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. Author manuscript; available in PMC: 2013 Sep 24.
Published in final edited form as: Chembiochem. 2012 Sep 7;13(14):2113–2121. doi: 10.1002/cbic.201200381

Figure 8.

Figure 8

Inactive compound and mutant reporter lack FRET response. A) Compound 6p, a structural analog of C646 that does not block p300 in vitro,[4] was analyzed for intrinsic fluorescence in untransfected cells under the same conditions as C646 and C107 in Figure 6. B) Cos7 cells were transfected with the Histac reporter and treated with 20 μM C107 or control compound 6p. C) Cos7 cells were transfected with either WT Histac or a Histac bearing a desensitizing point mutation in the bromodomain (Y65A). After 24 h, cells were imaged and treated with 40 μM C107.