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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Eur J Immunol. 2011 Jun 6;41(7):1832–1842. doi: 10.1002/eji.201041258

Figure 3. FACS and qPCR analysis of CD8+ Tregs.

Figure 3

(A) Expression of markers previously associated with Tregs on gated CD8+ T cells from responders (Resp, n=6) and non-responders (Non-resp, n=6) by flow cytometry after 5 day culture with Teplizumab. (B) Expression of NKG2A of both isoforms on CD8+ cells measured by RT-PCRfrom responders (n=5) and non-responders (n=3) to Teplizumab. RNA was extracted from sorted subpopulations of CD8+25+, CD25+25− T cells after activation with anti-CD3, or following culture with control IgG. The expression of both long and short isoforms were significantly different among the cell populations (*p<0.05, **p<0.01).