Figure 2. Hsp70 is induced in neurons and SCs.

DRG explant cultures were treated with vehicle, 1 μM KU-32 for 24 h, or subjected to 30 min HS plus a 1 h recovery (HS+R). Localization of Hsp70 expression (red) in neurons or SCs were examined using double-fluorescence-labelling with antibodies against PGP9.5 (A) (green) or S100β (blue) (B), respectively. Confocal images were taken and co-localization of Hsp70 with either PGP9.5 or S100β was performed using Image J. Arrows indicate increased Hsp70 in SCs. Scale bar, 15 μm.