Figure 4. KU-32 requires Hsp70 to block NRG1-induced demyelination.

DRG explants were established from C57Bl/6 (A, B) or Hsp70 KO (D, E) mice and myelinated in vitro for 3 weeks. The cultures were treated with vehicle or 1 μM KU-32 for 16 h prior to inducing demyelination with 200 ng/ml NRG1 for 3 days. Myelin internodes were labelled via MBP staining and nuclei visualized with a DAPI stain. Five to eight images were taken for each individual culture and the number of total and degraded myelin segments was counted per frame. Data shown are means±S.E.M. from three preparations per genotype. ***P<0.001 versus control; ˆP<0.01 versus NRG1 minus KU-32. (C) Explant cultures from WT mice were treated as above and lysates were prepared for immunoblot analysis of myelin protein zero (P0).