Figure 5. Hsp70 induction is sufficient to block NRG1-induced demyelination.

Fully myelinated WT or Hsp70 KO DRG explants were either uninfected or transduced with blank or Hsp70-FLAG adenoviruses for 16 h. The cultures were treated with vehicle or 200 ng/ml NRG for 3 days, stained for MBP and DAPI (A). The percentage of degenerated segments (B) and segment internode length (C) was quantified. Data shown are means±S.E.M. from three experiments per genotype. *P<0.05, **P<0.01, ***P<0.001 versus respective control; ˆP<0.05 versus blank+NRG1. Scale bar, 100 μm.