Figure 6. KU-32 inhibited NRG1-induced expression of c-Jun in WT but not Hsp70 deficient co-cultures.

(A) NRG1 treatment induces c-Jun in SCs. Fully myelinated WT DRG explants were treated with 100 ng/ml NRG1 for 16 h and stained for MBP and c-Jun levels. DAPI staining was used to visualize nuclei, scale bar, 10 μM. (B) Fully myelinated DRG explants from WT or Hsp70 KO mice were treated with vehicle or 1 μM KU-32 overnight then stimulated with 200 ng/ml NRG for 16 h. Cell lysates were prepared and the level of phospho-c-Jun and c-Jun were determined by immunoblot. The level of phospho-c-Jun (C) and c-Jun (D) were quantified using Image J and the data shown are means±S.E.M. from five experiments. **P<0.01 versus control in each genotype; ˆP<0.05 versus NRG1 in WT mice only.