Figure 8. KU-32 does not inhibit JNK or p42/p44 MAPK.

Myelinated WT cultures were treated with vehicle or 1 μM KU-32 and stimulated with 200 ng/ml NRG. Cell lysates were prepared and the levels of phospho JNK and total JNK (A) or phospho p42/p44 MAPK and total MAPK (B) were determined by immunoblot analysis. Cells were stimulated with NRG1 for 16 h or 45 min prior to preparing cell lysates to determine JNK and MAPK levels respectively. Gels shown are representative of results obtained in five experiments.