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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Proteomics. 2011 Aug;11(16):3326–3334. doi: 10.1002/pmic.201000445

Table 2.

Linear gradient for protein standard separation.

Time range % B
0–5 min 15% – 15%583
5–15 min 15% – 35%
15–20 min 35% – 40%584
20–25 min 40% – 40%
25–27 min 40% – 60%585