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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Eur J Immunol. 2011 Nov 10;41(12):3645–3655. doi: 10.1002/eji.201141708

Figure 5. Elevated BCR signaling reactions in Lyn-deficient B cells.

Figure 5

(A) Representative example of intracellular free calcium measurement of wild type B cells responding to anti-IgM stimulation at the time indicated by the arrow. The dashed line represents the level of fluorescence below which were 90% of wild type B cells of that subpopulation prior to stimulation. (B) Comparison of the fraction of B cells with spontaneous ex vivo intracellular calcium levels above the 90% threshold line as described in panel A (note: the wild type values are all 10% by definition). (C) Mean levels of phospho-Erk intracellular staining of wild type or Lyn-deficient B cells of different subsets (geometric mean fluorescence intensity). Experiments were repeated at least once with similar results. Statistical analysis: the values between the two genotypes were statistically different (unpaired t-test) at the following levels: + <0.05, * <0.01.