Skip to main content
. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Adv Mater. 2011 Dec 16;24(1):64–2. doi: 10.1002/adma.201103574

Figure 3.

Figure 3

Cellular encapsulation and in situ polymerization of PEG-4MAL hydrogel. A) Live/Dead staining of C2C12 murine myoblasts at 3 days after encapsulation in PEG hydrogels of varying polymer weight percentage compared to collagen-I gel (3 mg/mL). Cross-linked hydrogels could not be generated for low percentage PEG-4A and PEG-DA gels. Scale bar = 100 μm. Inset false color higher magnification showing individual cell spreading. MTS metabolic activity assay of encapsulated C2C12 cells indicates viability similar to number of cells seeded for PEG-4MAL and PEG-4VS, with PEG-4A and PEG-DA approximately 60%. B) H&E stain of PEG-4MAL matrix cross-linked directly on mouse myocardial wall. PEG-4MAL matrix incorporating 1% polymer substitution FITC-PEG-MAL cross-linked directly on mouse myocardial wall, counterstained with Alexa-Fluor 594 phalloidin and DAPI. Fluorescence intensity profiles for FITC-PEG-MAL and 594-phalloidin illustrate a physical incorporation depth of hydrogel into tissue of approximately 50 μm.