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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Chem Biodivers. 2012 Sep;9(9):1829–1839. doi: 10.1002/cbdv.201100320

Fig. 3.

Fig. 3

Left: Fabrication scheme for the assembly of the layered NO-releasing patches. Step 1, a 0.6 mm PDMS membrane is cast in a culture dish. Step 2, PDMS wells cast from 5 g of elastomer using a Teflon mold. Following curing, empty PDMS wells are pictured top, right. Step 3, the four quadrants are filled with cold 25% (w/w) Pluronic® F127 gel formulated for four experimental conditions. Top, left quadrant C1 contains Pluronic® F127 gel only. Top, right quadrant C2 contains 4 mg/g of 1 in Pluronic® F127 gel that has been exposed to light for 2 h, so all NO has been released. Bottom, right quadrant LD contains a low dose of 4 mg/g of 1 in Pluronic® F127 gel. Bottom, left quadrant HD contains a high dose of 8 mg/g of 1 in Pluronic® F127 gel. Step 4, once the gels warmed to room temperature and solidified, a thick layer of PDMS was layered over the Pluronic® F127 gel. Following curing, a finished sample is pictured bottom, right.