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. Author manuscript; available in PMC: 2013 Sep 3.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Aug 9;51(36):9075–9077. doi: 10.1002/anie.201203708

Figure 1.

Figure 1

Two molecular beacon (MB) probe-based strategies for the analysis of Rif-resistant Mtb. A) Currently used Expert MTB/RIF five MB probe approach.[5i-n] Absence of a signal from at least one MB probe ( e.g. MB5) indicates the presence of Rif-resistant Mtb DNA. B) Combination of YES and OR logic gates for the detection of Mtb and its resistance to Rif. The three DNA strands (C, D, and E) form a complex with UMB1 and the analyte to report the presence of Mtb DNA. A pair of strands M and F of the OR gate forms fluorescent complex with UMB2 and the analyte only if a mutation responsible for Rif-resistance is present in the sequence.