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The high frequency rR spectra of ferrous CO adducts of substrate-free wild-type CYP2B4 (A) and F429H mutant (B). Spectra were normalized to the glycerol mode at 1468 cm−1. The traces (C) and (D) are difference traces of ferrous CO adducts of CYP2B4 wild-type and 429H mutant in substrate-free form (C) and in BHT-bound state (D).
