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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: Proteomics. 2010 Nov 23;10(24):4415–4430. doi: 10.1002/pmic.201000298

Figure 3.

Figure 3

Selection of 7 ELISA bioimarker panel and validation of DIGE results. A. Goodness of separation analysis to select optimal biomarker panel size for the SJIA flare ELISA analysis. Using ELISA data from SJIA F/Q training and test data sets, as indicated, various classifiers of different panel size (feature #) were tested for their goodness of separation between flare (red) and quiescence (green) as shown by the box-whisker graphs. Boxes contain the 50% of values falling between the 25th and 75th percentiles; the horizontal line within the box represents the median value and the “whisker” lines extend to the highest and lowest values. B. ELISA assays validate biomarker observations from DIGE assays. The box-whisker graphs illustrate the spread of the protein abundance of each biomarker from SJIA F/Q, KD and FI samples using either DIGE or ELISA assays. Boxes contain the 50% of values falling between the 25th and 75th percentiles; the horizontal line within the box represents the median value and the “whisker” lines extend to the highest and lowest values.