. Author manuscript; available in PMC: 2012 Dec 7.

Published in final edited form as: Proteomics Clin Appl. 2009 Oct 13;3(12):1440–1450. doi: 10.1002/prca.200900020

Table 3.

Activation of the signal effectors by PMA treatment only

	Ratio ^a
4E-BP1(Thr36)	1.15
4E-BP1(Thr45)	6.65
Akt(Ser473)	1.37
FOXO4(Ser197)	1.52
Gab1(Tyr627)	1.59
HSP27(Ser82)	1.49
IKK α(Thr23)	1.29
IRS-1(Ser307)	2.73
IRS-1(Ser639)	1.11
JNK(Thr183)	1.56
PTEN(Ser380/Thr382/Thr383)	1.97
Raf1(Ser259)	1.18

For each treatment group, the phosphorylation signal ratio induction was calculated and further normalized by the ratio obtained from the untreated, control group, which was given a value of 1. The coefficients of variation for the fluorescence intensity are ≤ 6% from replicate spots and arrays; the CVs of phosphorylation signal ratio induction are therefore ≤ 12 %. The statistical analysis shows that the ratio change between PMA treatment and the control is significant with p < 0.05.