Construct design for the production of HBsAg in Zea mays. glb1, 1.4kb globulin1 promoter; 3kbglb1, extended globulin1 promoter; 3xglb1, tandemly repeated extended globulin1 promoter; BAASS, barley alpha amylase signal sequence; Vac, Vacuolar targeting sequence; HB, hepatitis B surface antigen; PinII, potato proteinase inhibitor II termination sequence. All constructs also contained an herbicide resistance gene following the PinII termination sequence.