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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Plant Biotechnol J. 2012 Jul 21;10(8):979–984. doi: 10.1111/j.1467-7652.2012.00727.x

Figure 4.

Figure 4

Effect of oil extraction and temperature on maize-produced HBsAg, as determined by immunoblot. Arrowhead indicates monomeric form of HBsAg. L, prestained protein ladder; Std, 100ng yeast-derived rHBsAg standard; Neg ctrl, non-transgenic maize material; FF, full fat HBsAg maize; SFE, supercritical fluid extracted maize; Hex, hexane extracted maize. All maize samples were loaded at 1.5μg of total protein with the exception of the full fat sample treated at 80°C which was loaded at a maximum well volume (equivalent to 0.5μg). This corresponds to 0.2 to 0.3mg of starting maize material in lanes 3 through 9 and 0.5mg in lane 10.