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. 2012 Oct 9;24(10):3967–3981. doi: 10.1105/tpc.112.103044

Figure 2.

Figure 2.

Cytokinin Response in the Pericycle Cells.

(A) and (B) TCS:GFP signal in pericycle cells of 7-d-old roots without treatment (A) and on media supplemented with 10 μM NPA (B).

(C) and (D) TCS:GFP expression in pericycle cells of 3-d-old roots without LRPs (C) and 4-d-old roots with one or two initiated LRPs. Weak TCS signal present in xylem pole pericycle cells and in the endodermis adjacent to LRP (D).

(E) Cytokinin measurements in roots, excluding the root meristem of seedlings grown on control medium (white) and in the presence of 10 μM NPA (black). Error bars denote se (*P < 0.05; Student’s t test; n = 3).

(F) Real-time monitoring of TCS:GFP x DR5:3XVENUS-N7 reporter in pericycle cells after LRP initiation. White asterisks and yellow arrowheads indicate LRPs at the early initiation stage in pericycle cells visualized by nuclear-localized DR5:3XVENUS-N7 and the TCS:GFP signal, respectively.

(G) and (H) Defective LRI positioning in 7-d-old seedlings of the arr1 arr11 mutant. Proportion of misplaced per total number of LRPs (wild type, n = 218; arr1-3 arr11-2, n = 267) from 18 roots each. Error bars denote se (*P < 0.05; Student’s t test) (G). One pericycle cell distance between two LRPs (H).

(I) Auxin-induced LRP initiation in arr1 arr11 and ipt3 ipt5 ipt7 mutants. In the mutant background, 25 h of treatment with 1 μM 1-naphthaleneacetic acid (NAA) induces LRP initiation along the whole root, whereas in control roots, induction is restricted mainly to the LRP initiation zone. Error bars denote se (*P < 0.05; Mann-Whitney test; n = 15 roots).

White asterisks indicate borders of LRPs. cZ, cis,zeatin; cZ9G; cis-zeatin-9-glucoside; cZMP, cis-zeatin riboside monophosphate; cZOG, cis-zeatin-O-glucoside; cZR, cis-zeatin riboside; cZROG, cis-zeatin riboside-O-glucoside; DHZ, dihydrozeatin; DHZ9G, dihydrozeatin- 9-glucoside; DHZMP, dihydrozeatin riboside monophosphate; DHZOG, dihydrozeatin-O-glucoside; DHZR, dihydrozeatin riboside; DHRZOG, dihydrozeatin riboside-O-glucoside; DHZR, dihydrozeatin riboside; Em Z, zone of LRP emergence; iP, isopentenyladenine; iP9G; isopentenyladenine-9-glucoside; iPMP, isopentenyl adenosine monophosphate; iPR, isopentenyl adenosine; LRP Z, zone of LRP initiation; RM, root meristem; tZ, trans-zeatin; tZ9G, trans-zeatin-9-glucoside; tZMP, trans-zeatin riboside monophosphate; tZOG, trans-zeatin-O-glucoside; tZROG, trans-zeatin riboside-O-glucoside; tZR, trans-zeatin riboside; wt, the wild type. Bars = 50 μm.