Table 3. Phenotypic Classes of zix Alleles 1.5 DAP.
Genotype | zy+(1-4end) | zy+(6-8end) | ez+(2-4end) | ez+(6-12end) | 1c+(8-16end) | 2c | unf | pn | n |
---|---|---|---|---|---|---|---|---|---|
Ler wild type s | 24.2% | 3.6% | 4.6% | 59.4% | 3.1% | 4.1% | 0.5% | 0.5% | 322 |
zix-1/ZIX s | 41.6% | 3.1% | 6.2% | 34.2% | 2.5% | 3.1% | 7.5% | 1.8% | 322 |
zix-1/ZIX c | 38.7% | 8.5% | 8.3% | 27.1% | 2.6% | 3.9% | 9.7% | 1.8% | 362 |
zix-2/ZIX s | 56.8% | 6.7% | 5% | 19.4% | 0.6% | 0 | 11% | 0.4% | 315 |
zix-2/ZIX c | 50.5% | 6.9% | 4.5% | 30% | 0.7% | 0 | 8.2% | 0 | 291 |
zix-3/ZIX s | 41.5% | 9% | 5.4% | 35.5% | 1% | 1.6% | 5.4% | 0.6% | 299 |
zix-3/ZIX c | 44.6% | 9.8% | 4.2% | 32.2% | 0 | 0.3% | 7.4% | 1.4% | 285 |
Two days after emasculation, stage 12 zix/ZIX flowers were manually pollinated with self or wild-type pollen and the pistils were dissected 1.5 d later. Whole-mount ovules and seeds were cleared with chloral hydrate and scored under a DIC microscope. s, selfed; c, crossed with the wild type; :n, numbers of ovules from five to six siliques; zy, early symmetric zygote; end, endosperm nuclei; ez, elongated zygote; 1c, one-cell embryo; 2c, two-cell embryo; unf, unfertilized ovules; pn, unfused polar nuclei.