Table 4. Phenotypic Classes of zix Alleles at 3 DAP.
| Genotype | glob | ea.gl/8c | 4c/2c/1c | unf | 8c.m/4c.m/2c.m | 1c.m/zy.m | col | n |
| Ler wild type s | 23 | 96/38 | 27/25/5 | 1 | 0/0/0 | 0/0 | 14 | 229 |
| 10% | 58.5% | 25% | 0.5% | 0% | 0% | 6% | 100% | |
| zix-1/ZIX s | 0 | 151/19 | 10/4/1 | 10 | 3/5/5 | 46/21 | 22 | 297 |
| 0% | 57.2% | 5% | 3.3% | 4.4% | 22.7% | 7.4% | 100% | |
| zix-1/ZIX c | 7 | 105/37 | 27/3/1 | 5 | 6/5/4 | 30/11 | 19 | 260 |
| 2.7% | 54.6% | 11.9% | 1.9% | 5.8% | 15.8% | 7.3% | 100% | |
| zix-2/ZIX s | 0 | 68/52 | 29/16/5 | 20 | 1/2/11 | 49/26 | 21 | 300 |
| 0% | 40% | 16.6% | 6.7% | 4.7% | 25% | 7% | 100% | |
| zix-2/ZIX c | 1 | 81/58 | 18/20/3 | 8 | 0/5/10 | 32/5 | 21 | 262 |
| 0.4% | 53% | 15.7% | 3.1% | 5.8% | 14% | 8% | 100% | |
| zix-3/ZIX s | 2 | 64/37 | 18/14/4 | 24 | 0/1/4 | 50/27 | 20 | 265 |
| 0.8% | 38.1% | 13.6% | 9% | 1.9% | 29% | 7.6% | 100% | |
| zix-3/ZIX c | 0 | 77/52 | 16/11/5 | 13 | 0/7/11 | 57/7 | 16 | 272 |
| 0% | 47.4% | 11.8% | 4.8% | 6.6% | 23.5% | 5.9% | 100% |
Two days after emasculation, stage 12 zix/ZIX flowers were manually pollinated with self or wild-type pollen and the pistils were dissected 3 d later. Whole-mount ovules and seeds were cleared with chloral hydrate and scored under a DIC microscope. The percentage of each class or underlined group is given underneath the class or group. s, selfed; c, crossed with the wild type; n, numbers of ovules from five siliques; glob, globular stage; ea.gl, early globular stage; 8c, eight-cell stage; 4c, four-cell stage; 2c, two-cell stage; 1c, one-cell stage; unf, unfertilized ovules; 8c.m, eight-cell mutant; 4c.m, four-cell mutant; 2c.m, two-cell mutant; 1c.m, one-cell mutant; zy.m, zygotic mutant; col, collapsed ovules. The 1c.m mutant class had a one-cell embryo with nondividing suspensor and ≤8 endosperm nuclei. 2c.m, 4c.m, and 8c.m mutant classes had greater than or equal to twofold reduction in endosperm nuclei compared to wild-type seeds at the same stage.