Figure 5.

Representative Arabidopsis Leaf and Root Organic Acids and Ions Exhibiting Differences in Concentration between the gdh1-2-3 Mutant and the Wild Type Following Prolonged Darkness.

T0 = plants grown under short-day conditions and harvested 2 h after the beginning of the light period, D3 = after three continuous days in the dark, and D7 = after seven continuous days in the dark. The dotted line corresponds to the gdh1-2-3 mutant and the solid line to the wild type. The data for leaf and root metabolomic analyses are presented in Supplemental Data Sets 1 and 2 online and were obtained as described in Methods. The metabolite content is expressed in arbitrary units, which correspond to normalized values of the peak area⋅standard ribitol⁻¹⋅leaf dry weight⁻¹. Bars indicate se. Magnification of the scale is shown when there were significant differences between the wild type and the mutant at T0 and T3. The name of the metabolite is in a white box when differences occurred both in the roots and leaves and in a pale-gray or dark-gray box when either in the leaves or roots, respectively. Results are presented as mean values for five plants with se. Asterisks indicate a bilateral t test P value <0.01 (**) and <0.05 (*) for statistically significant differences.