Figure 8.

Differences in the Spectrum of Amino Acids in the Wild Type and gdh1-2-3 Mutant in the Dark.

In the left panels, differences in the total free amino acid content are shown. T0 = plants grown under short-day conditions and harvested 2 h after the beginning of the light period, D3 = plants harvested after three continuous days in the dark, D7 = plants harvested after seven continuous days in the dark. The red line corresponds to the gdh1-2-3 mutant and the blue line to the wild type (WT). Leaves (A) and roots (B). In the right panels, the main classes of amino acids accumulated after seven continuous days of darkness are shown. The amino acids derived from 3-phosphoglycerate (3PGA) are Cys, Gly, and Ser, from phosphoenolpyruvate (PEP) are Phe, Trp, and Tyr, from pyruvate are Ala, Leu, and Val, from oxaloacetate (OAA) are Asp, Asn, Ile, Lys, Met, and Thr, from 2-oxoglutarate are Arg, GABA, Gln, Glu, and Pro, from the urea cycle are Cit and Orn. Metabolite content is expressed in arbitrary units, which correspond to normalized values of the peak area⋅standard ribitol⁻¹ leaf dry weight⁻¹. Error bars correspond to se.