Table 1. Primary Metabolites Exhibiting an Increase or a Decrease in Roots and Leaves of the gdh1-2-3 Mutant Compared to the Wild Type and Following Prolonged Darkness.

Organ T0				Organ D3				Organ D7
Leaves				Leaves				Leaves
Increased	FC	Decreased	FC	Increased	FC	Decreased	FC	Increased	FC	Decreased	FC
Glcc,e	3.2	Gly	0.5	Glcc,e	3.1	2-Oxoglutarated,e	0.6	Alac,e	3.6	2-Oxoglutarated,e	0.4
Fruc,e	2.1	2-Oxoglutarated,e	0.6	Alac	1.9	Cys	0.7	Aspc	3.6	Gln	0.5
His	1.7	Gln	0.7	Aspc,e	1.8			GABAc	2.3	Pyruvate	0.5
Pyruvate	1.7	Glc-6-P	0.7	Fruc,e	1.6			Pro	2.0	Fru-6-P	0.6
Suc	1.6	Aspd	0.7	Succinate	1.4			Glcc,e	1.9	Glc-6-P	0.7
GABAc	1.4			Fumaratec,e	1.4			Ser	1.7
Pro	1.3			Malate	1.3			Fruc,e	1.7
Fumaratec,e	1.3			GABAc,e	1.2			Tyr	1.6
Alac,e	1.3							Leu	1.5
								Lys	1.4
								Fumaratec,e	1.4
								Thr	1.3
								Ile	1.3
								Val	1.3
Roots				Roots				Roots
Increased	FC	Decreased	FC	Increased	FC	Decreased	FC	Increased	FC	Decreased	FC
GABAc,e	2.3	Aconitate-trans	0.6	GABAd,e	6.6	2-Oxoglutarated	0.4	Alac,e	9.2	2-Oxoglutarated	0.1
Fumarate	1.8	Citrate	0.7	Alac,e	5.8	Malated	0.5	GABAd,e	6.9	Citrate	0.1
Pro	1.7			Aspc	4.3	Fru-6-Pd	0.7	Aspc	4.4	Fru-6-Pd	0.1
Suc	1.5			Leuc	2.6	Succinate	0.7	Ilec,e	2.6	Glc-6-Pd	0.1
Leu	1.5			Lysc	2.2	Glc-6-Pd	0.7	Valc,e	2.2	Malated	0.1
Trp	1.5			Ilec,e	1.8			Leuc	1.9	Gln	0.2
Phe	1.5			Fumarate	1.6			Serc	1.8	Phe	0.2
Ilec,e	1.3			Glyc	1.5			Pyruvatec	1.8
Alac,e	1.3			Pyruvatec	1.5			Asn	1.7
Valc,e	1.3			Valc,e	1.4			Glyc	1.5
				Glu	1.4			Glc	1.3
				Fru	1.4
				Suc	1.3
				Serc	1.3

Metabolomic analysis was performed on roots and leaves of plants grown under hydroponic conditions and harvested at the rosette stage as described in Methods. T0 = plants grown under short-day conditions and harvested 2 h after the beginning of the light period, D3 = after 3 d in the dark, and D7 = after 7 d in the dark. Fold change (FC) corresponds to the ratio of gdh1-2-3 mutant/wild type calculated by taking the average of the metabolite concentrations measured in the replicates of the metabolomic analyses (see Supplemental Data Sets 1 and 2 online). Those exhibiting a similar pattern of accumulation in both roots and leaves whatever the time point of the experiment are in bold characters. A schematic representation of the accumulation pattern of a number of selected metabolites exhibiting the most characteristic changes in roots after dark exposure is presented in Figure 9. The – indicates where the number of compounds is different from one column to the next.

^aIncreased fold change (FC > 1.25).

^bDecreased fold change (FC < 0.75).

^cMetabolites present in higher concentration in the leaves of the mutant at T0, D3, and D7.

^dMetabolites present in higher concentration in the roots of the mutant at T0, D3, and D7.

^eMetabolites exhibiting a similar pattern of accumulation in roots and leaves at the three time points of the experiment.