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. 2012 Oct 26;24(10):4083–4095. doi: 10.1105/tpc.112.104620

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 4. — RBR1 Binds to Cell Cycle Genes and Genes Involved in Cell Differentiation in the Root.

(A) ChIP of Pro_RBR1:RBR1:RFP seedlings using an anti-RFP antibody. The promoter regions of At4G01330, MGP1, SCL3, SCR, and the cell cycle genes PCNA1 and MCM5 are bound by RBR1, whereas the heterochromatic regions (RB32.5 and RB45) show no difference between mock and treatment.

(B) RBR1 targets MCM5 and PCNA1 can also be detected with an E2FA antibody. However, no enrichment could be observed for the SHR targets tested in (A).