Figure 3.

Downregulation or Overexpression of Arabidopsis XRCC4 Results in Increased or Decreased Agrobacterium-Mediated Transformation Efficiencies, Respectively.

(A) Root segments from wild-type Col-0, representative XRCC4 RNAi lines (top panel), and overexpression lines (bottom panel) were infected with tumorigenic strain Agrobacterium A208 and photographs were taken 4 weeks after infection.

(B) Frequency of tumor or transgenic calli formation in XRCC4 RNAi lines after challenging the root segments with Agrobacterium A208 for the tumorigenesis assay or disarmed Agrobacterium GV3101 harboring pCAS1 for the stable transformation assay that produces PPT-resistant calli.

(C) Efficiency of tumor or transgenic calli formation in XRCC4 overexpression lines. In (B) and (C), data are presented as mean ± sd (n = 3) from three replicates. Asterisks indicate a significant difference between Col-0 and XRCC4 RNAi or overexpression lines according to Student’s t test (P < 0.05; see Supplemental Figures 3 and 4 online).