Figure 4.

Correlation of Pyrethrin Product Accumulation and Gene Expression across All Stages of Achene Development.

Pyrethrin (Py) content was measured in whole flowers of stages 1 to 5 (S1 to S5). For the final stages 6 and 7, dry seeds, embryos, and pericarps were microdissected and separately examined for pyrethrin content. Peak areas were determined using specific masses 123 or 133. For the graph, stages 5 and 6 were matched to have the same relative content, but stage 6 will be somewhat higher. Transcript levels of CDS, FDS1, and GLIP were measured in dissected ovaries stages 2 to 7 and normalized against GAPDH. All data were determined in triplicate. se error bars were left out for most data for better visualization.