The Terpene Precursors of Pyrethrin Biosynthesis Are Synthesized in Trichomes and Transported to the Pericarp.
(A) Relative gene expression of CDS, FDS1, and GLIP in ovaries with trichomes (O+), ovaries with 85% fewer trichomes (O-), and isolated glandular trichomes (T) after normalization against GAPDH expression. Lowercase letters indicate significant differences for that gene relative to the other tissues (P < 0.05).
(B) The relative content of precursors COH and CA, and pyrethrins (Py; right y axis) that could be traced to be specifically contained in trichomes and not in pericarp (see Supplemental Tables 2 and 3 online; peak areas of specific masses were integrated per compound). Lowercase letters indicate significant differences. Error bars represent se (n = 3).