Table 1. Specific Chrysanthemyl Diphosphate Synthase Activity in Crude Protein Extracts of Seedlings, Ovaries, and Trichomes.

		COHc	Trichome Lactonesd	Proteine	Specific CDS Activityf
Tissuea	Treatmentb	(area/µL × 1000)	(area/µL × 1000)	(ng/µL)	(area/ng)
Seedling	Control	0	0
	DMAPP	0	0
	De novo	0 (0%)	(0%)	114	0
Ovaries with trichomes	Control	42 ± 1.6	712 ± 166
	DMAPP	211 ± 27	537 ± 212
	De novo	169 (100%)	(100%)	176	193
Ovaries with fewer trichomes	Control	9.2 ± 4.8	229 ± 76
	DMAPP	70 ± 0.8	178 ± 67
	De novo	61 (36%)	(32%)	132	93
Glandular trichomes	Control	9.9 ± 4.8	130 ± 62
	DMAPP	51 ± 18	110 ± 104
	De novo	41 (24%)	(20%)	2.80	2973

^aCDS activity was based on extracts of 250 mg tissue. Glandular trichomes were removed from 250 mg ovaries with ∼68% efficiency and recovered with ∼20% efficiency, column 4.

^bIn the treatments, “De novo” refers to the subtraction of COH content of controls from DMAPP-incubated extracts

^cCOH peak areas of specific mass 123 are given (±sd, n = 3). Relative total CDS activity is given in parentheses.

^dTrichome lactones are peak areas of specific mass 83 (±sd, n = 3) of STL compound E and representative of trichome numbers in various tissue preparations. Calculated relative trichome numbers are given in parentheses, averaged for control and DMAPP.

^eProtein concentrations are given per tissue type and are the same per treatment.

^fSpecific CDS activity is only given for de novo–produced chrysanthemyl.