Figure 1.

Isolation of cp29a Null Mutants.

(A) Gene map of CP29A (At3g53460) with four exons (black bars) and three introns (black lines). Positions of the T-DNA insertions cp29a-1 (SALK_003066) and cp29a-6 (001G06) are marked by triangles.

(B) Immunoblot analyses using anti-CP29A and anti-CP31A antibodies demonstrate the absence of CP29A and CP31A in cp29a and cp31a single mutants, respectively, and the lack of both proteins in cp29a × cp31a double mutants. A quantitative analysis with dilutions of wild-type protein extracts demonstrates that loss of CP29A does not affect the accumulation of CP31A and vice versa (see Supplemental Figure 1 online).