Figure 2.

Substrate Preferences and Interaction Specificities of the Wild Type and CA-MAPKs.

(A) Phosphorylation of the semidegenerate peptide array by MPK6^Y144C and MPK6^D218G/E222A.

(B) and (C) Combinatory interaction in yeast two-hybrid assays of wild-type (WT) and CA forms of MPK3, 4, and 6 with MKK2 and MKK4 (B) and with VIP1, MKS1, and ERF104 (C). Cotransformed single yeast colonies were spotted on control medium (c.) and selective medium (i.) supplemented with 65 mM (B) or 100 mM 3-amino-1,2,4-triazole (C).