Figure 3.

Characterization of Arabidopsis mpk4-2 Mutants Complemented with CA-MPK4 Loci.

(A) Morphological aspect of mpk4-2/mpk4-2 lines complemented with MPK4^WT and MPK4^D198G/E202A. Plants were grown in short days for 5 weeks. K4WT and K4DE are abbreviations for mpk4 lines complemented with pGREEN0229-MPK4L-PC2 and pGREEN0229-MPK4L^D198G/E202A-PC2, respectively. Numbers 1 to 4 refer to line numbers.

(B) and (C) Kinase activity toward MBP of MPK4-myc immunoprecipitated from mpk4-2/mpk4-2 lines complemented with MPK4^WT ([B] and [C]), MPK4^Y124C (B), and MPK4^D198G/E202A (C). K4Y (B) is an abbreviation for mpk4 lines complemented with pGREEN0229-MPK4L^Y124C-PC2.

(D) Kinase activity of K4WT and K4DE lines upon 15 min flg22 (1 µM) treatment.

(E) Morphological phenotype of 5-week-old mekk1-1/mekk1-1 MPK4^D198G/E202A plants grown in long days. mekk1-1/mekk1-1 plants barely survive 2 weeks in pots.

[See online article for color version of this figure.]