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. 2012 Oct 31;24(10):4281–4293. doi: 10.1105/tpc.112.101253

Figure 6.

Figure 6.

Characterization of RPS4-Mediated ETI in CA-MPK4 Lines.

(A) Growth of Pst DC3000 AvrRps4 in the indicated genotypes. Bacterial titers were determined 2 h (day 0, dark-gray bars) and 3 d (light-gray bars) after spray inoculation. Bars are average of at least four replicates, and error bars show sd. *P value < 0.05 and **P value < 0.01. Similar results were obtained in at least three independent experiments.

(B) Disease symptoms observed on K4WT and K4DE lines 3 d after inoculation with Pst DC3000 AvrRps4.

(C) SA content before (T0) and 24 h after spray inoculation with Pst DC3000 AvrRps4 or after mock treatment with 10 mM MgCl2. Bars represent the average of three independent biological replicates. After Pst DC3000 AvrRps4 treatment, the SA content of Col-0 and wild-type lines taken together was 8.2 ± 1.0 ng⋅mg−1 fresh weight (FW) (n = 9) and of K4DE lines was 5.1 ± 1.0 ng⋅mg−1 fresh weight (n = 9) (the difference is statistically significant; P value < 0.05).