Figure 4.

Pst DC3000/avrRpt2–Induced Kinetic Expression of 12 Defense Genes in med16/sfr6 Mutants.

Plants were inoculated with the avirulent bacterial pathogen Pst DC3000/avrRpt2 (OD₆₀₀ = 0.001). Leaf tissues were collected at the indicated time points. Total RNA was extracted from the inoculated leaves and analyzed for the expression of indicated genes using real-time qPCR. Expression was normalized against constitutively expressed UBQ5. Data represent the mean of three independent samples with sd. An asterisk (*) indicates that the expression level of the gene in the wild type (WT) was either significantly lower (WRKY38 and WRKY62) or significantly higher (DIR1, AZI1, PR1, and PR5) than in both med16-1 and sfr6-2 (P < 0.05, Student’s t test). The experiment was repeated with similar results.