Figure 4.  .

Forskolin treatment decreases vessel stability in Mrp4-knockout mice. (A) Whole-mount staining for PECAM (green) and NG2 (blue) showing the degree of pericyte coverage in the proximal retina of forskolin- or DMSO-treated P6 mice. (B) Quantification of the number of NG2-positive cells per EC area. P value is <0.01 in comparison between forskolin-treated Mrp4^−/− mice and any other group (*). (C) Whole-mount staining of PECAM (green) and type 4 collagen (Col4) (red) in forskolin- or DMSO-treated P6 retinas. Empty sleeves (Col4+, PECAM−) are observed in the proximal vascular plexus (arrows). (D) Quantification of the number of empty sleeves per EC area. P value is <0.01 in comparison between forskolin-treated Mrp4^−/− mice and any other group (*). A, artery; V, vein. The data are provided as the mean ± SD.