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Brightfield (a,b) and darkfield (c,d) images of cells incubated with either freshly prepared GNP-PEG (a,c) or GNP-PEG pre-incubated in media for 2 days (b,d). Nanoparticles appear red in brightfield transmittance mode and green to orange in darkfield reflectance mode. Scale bar is ca. 20 um.
