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Western blot showing the level of vascular endothelial growth factor (VEGF) in MDA-MB-231 cells was dramatically reduced following the treatment of MSeA at 5 μM for 12 hrs. A higher MSeA concentration (10 μM) or longer treatment period (24 hrs) did not further inhibit the VEGF production. Commercial antihuman VEGF antibody (1: 200XD) was used to visualize the cellular VEGF protein band at the given MSeA concentrations and treatment time periods.
