Table 1.

Genes associated with ceramide metabolism in C. gigas from the in silico search

GeneID	Gene description	Species	e-value
Cg_4852	Serine palmitoyltrasnferase-1	Pongo abelii	0
Cg_877	Acid ceramidase	Rattus norvegicus	2E-146
Cg_14141	3-ketodihydrosphingosine reductase	Mus musculus	5e-46
Cg_29918	Ceramide glucosyltransferase	Xenopus tropicalis	9e-122
Cg_21728	Acid sphingomyelinase	Mus musculus	5e-46
Cg_16356	Ceramide kinase	Homo sapiens	1e-27
Cg_16726	Cerebrosidase	Mus musculus	5e-139
Cg_17230	Neutral ceramidase	Oryza sativa	5e-96
Cg_1560	Caspase 7	Homo sapiens	4e-10
Cg_23531	Caspase 8	Homo sapiens	8e-54
Cg_252	TNF receptor-associated factor 2	Mus musculus	1e-53
Cg_3248	TNF receptor-associated factor 3	Mus musculus	2e-30
Cg_31180	TNF receptor-associated factor 4	Homo sapiens	4e-39
Cg_6808	Neutral sphingomyelinase	Caenorhabditis elegans	4e-10
Cg_20643	Dihydrosphingosine 1-phosphate phosphatase	Schizosaccharomyces pombe	3e-9
Cg_26221	Sphingosine-1-phosphate phosphatase	Mus musculus	8e-20
Cg_7888	Sphingosine-1-phosphate lyase	Dictyostelium discoideum	6e-9
HS213433	Sphingomyelin synthase	Homo sapiens	1e-100
HS185280	Ceramide synthase	Mus musculus	5e-83
HQ425701	Inhibitor of apoptosis	Crassostrea gigas	-
HQ425703	Caspase 1	Crassostrea gigas	-
HQ425705	Caspase 2	Crassostrea gigas	-
HQ425699	Fas-associated receptor with Death Domain	Crassostrea gigas	-

Genes associate with ceramide metabolism were identified in C. gigas by searching of publicly available databases. Sequences assembled from short read archive data and expressed sequence tags were given a Gene ID code that corresponds to the sequence in the Additional file 1. For each of these contiguous sequences, the top BLASTx hit description, corresponding species, and e-value are provided. Two genes that matched to a single EST each in GenBank (sphingomyelin synthase and ceramide synthase) are denoted with their respective GenBank Accession Numbers since they are not represented as a contig of multiple ESTs. An additional four genes [GenBank: HQ425699, HQ425701, HQ425703, and HQ425705] have been previously characterized in C. gigas[20].