Table 3.
Sequencing and qPCR primers for ceramide metabolism genes
| Gene description | Elongation factor 1 α (EF1α) | Serine palmitoyltransferase (Sptlc1) | 3-ketodihydrosphingosine reductase (3KDSR) | Acid ceramidase (AC) | Glucosylceramide synthase (GlcCer) |
|---|---|---|---|---|---|
| Sequencing Forward Primer |
- |
ATGGCGTCGACGTTCA TTCC |
AGCGACGGACCGAACTTACT |
TGTGATTACACGAT GTGGATACCG |
AGAGGCGAACACA CGAAAGT |
| Sequencing Reverse Primer |
- |
CTGTTCCCCAATATTTC TGAC |
TGTCTTGGGTTTTGCATCCTTC |
CTGCTTCTGACTTC CGGTGT |
CCATATGGATAACAC TTCTCG |
| Sequence Product Size (bp) |
- |
1483 |
1210 |
1165 |
1080 |
| qPCR Forward Primer |
AAGGAAGCTGCTGA GATGGG |
TTCACAGCAAGCT GAGCGAT |
GCAGTGCAGTGGCTGGAAAT |
TGGACTCAAGTTGG CCAGGA |
TTGGCCCAAACGGG AAAGTT |
| qPCR Reverse Primer |
CAGCACAGTCAG CCTGTGAAGT |
AAGTAGCGAGCC AACGTCAC |
AGGCAGCCTTGGTGACATTG |
AAGGCTGGGGGAG AGTATCG |
TGTCCATGAGCGAGT CTGGT |
| qPCR Product Size (bp) | 200 | 178 | 168 | 157 | 114 |